MUC1 anticorps (FITC)

N° du produit ABIN119389

Cet anticorps Souris Monoclonal détecte spécifiquement MUC1 dans FACS et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour MUC1 anticorps (FITC) (ABIN119389)

Antigène: MUC1 (Mucin 1 (MUC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC1 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C595

Détail du produit anti-MUC1 anticorps (FITC)

Specificité: This antibody recognises CD227, the breast cancer associated mucin encoded by the Muc-1 gene. In normal tissues expression is restricted to specialised glandular epithelial cells. Clone C595 recognises the peptide epitope ARG-PRO-ALA-PRO within the protein core of the Mucin.

Purification: Affinity Chromatography on Protein G

Immunogène: Urinary MUC-1 mucin. Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line.

Isotype: IgG3

Information d'application

Indications d'application: Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Aziden as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MUC1

Antigène: MUC1 (Mucin 1 (MUC1))

Autre désignation: CD227 / Mucin-1 / MUC1

Sujet: MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.Synonyms: Breast carcinoma-associated antigen DF3, CA 15-3, Carcinoma-associated mucin, DF3, EMA, Episialin, H23AG, MUC-1, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin

ID gène: 4582

NCBI Accession: NP_001018016

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling